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Fluorescent EVs from HEK293 cells (CD63-EGFP)

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Lyophilized EGFP (green) Fluorescent EVs from HEK293 cells (CD63-EGFP)

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HBM-HEK-EGFP63
€299.00

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Product Overview

FLUO-EVs are stably-fluorescent extracellular vesicles expressing the fluorescent protein EGFP (green) as fusion protein with tetraspanins CD9, CD81 and CD63. FLUO-EVs are purified with a combination of tangential flow filtration (TFF) and size exclusion chromatography (SEC) from genetically engineered cells in order to express the fluorescent proteins as fusion protein with the tetraspanin CD9, CD63 or CD81. FLUO-EVs demonstrated high stability of the fluorophores, they can be used for in vitro tracking studies or as reference material for analyzers of nanoparticles or for assay calibration.

 

Characteristics: Particle size distribution 50 – 140 nm, lyophilized, storage at 4-8 °C.

Applications: Positive control for EV analyzers (NTA, flow cytometers, fluorescence microscopy), cell spike-in and in vitro tracking, EV uptake monitoring, assay and instrument calibration.

Origin: human embryonic kidney (HEK293 cells)
Quantity per vial: 1x10^9 fluorescent particles.
Storage of lyophilized exosomes: Store up to 1,5 years at 4°C (Expiration date indicated on the label).
Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.
Spectrum data: Excitation peak 488 nm Emission peak 507 nm

Control (spike-in) for exosome quantification.

Fluorescence assay calibration

Flow Cytometry

NTA in Fluorescent Mode

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