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Exo-FACS ready to use kit for analysis of human SERUM exosomes

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Exo-FACS Ready to Use Kit for FACS analysis.

Standard
HBM-FACS-PES
€360.00

Price excludes VAT

Product Overview

Exo-FACS allows exosome isolation from biofluids or cell culture media and FACS analysis of exosome markers. The kit consists of EXO-Prep reagent for exosome isolation, 4 µm beads used for the overall capture of pre-isolated exosomes, and lyophilized exosomes from cell culture supernatants or human biological fluids as positive control. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens. HBM offers different Exo-FACS kits for staining of exosome markers from human biofluids (plasma, urine, serum, saliva) and from cell culture supernatants. Exo-FACS contains reagents for 20 reactions (lyophilized exosomes, beads, antibodies and buffers). Primary antibody included in the kit is against a common exosome marker (CD9 or CD63) and can be used as a positive control for protein profiling via FACS analysis.

 

Applications: Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.

Advantages: Ready to use. No initial exosome purification required. Lyophilized Exosome Standards for positive control included. User friendly and suitable for multiple marker analyses.

Sample type: Serum
Starting material: Exo-FACS contains reagents and antibodies for 20 reaction. Exosome standards must be reconstituted in 100 µl of deionized water. Beads are ready to use for exosome capture. Primary and secondary antibody must be appropriately diluted in sample buffer.
Assay type: Kit allows fast and easy detection of exosomal markers in a set of purified exosomes (choosen by customer) via FACS. Kit provides a proprietary antibody against a common exosomal marker as positive control.
FACS-Beads: 4 µm Aldheyde-Sulfate latex beads (100 µl).
Antibody: rimary antibody for exosome marker detection as positive control, Anti-CD9. Secondary antibody Alexa 488.
Exosome standard: 1 vial (100 µg) of HBM-exosome standards (lyophilized), from human serum (number of particles/ml 1x10^10).

FACS

Exosome isolation and exosome marker characterization via FACS.

Exosome comprehensive profiling

All documents are provided in PDF format for easy viewing and printing. For additional technical documentation, please contact our support team.

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