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Lyophilized Exosomes from Urine of Healthy Donors (30µg)

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Lyophilized Exosomes from Urine of Healthy Donors (30µg)

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Product Overview

Purified urine exosomes (small EVs) are obtained from a pool of certified healthy donors. Exosomes/small EVs (40–120 nm) are purified using a double tangential flow filtration process using HBM-LS proprietary filters TFF-MV and TFF-EVs. Purified EVs are validated and characterized for overall protein content, marker expression (CD9, CD63, CD81), particle concentration and size distribution (Nanoparticle Tracking Analysis performed with the ZetaView® Evolution analyzer, Particle Metrix), and morphology (TEM).

The effects of lyophilization on EV protein stability are comparable to other storage methods, including storage at −80 °C. Lyophilized vesicles are easy to store and ship, and remain stable long-term for up to 36 months.

 

Applications: Control (spike-in) for EV quantification, protein marker analysis (ELISA, WB, flow cytometry). Extraction and analysis of EV associated nucleic acids (qPCR, ddPCR, RNA seq). NTA, fluorescent NTA (F-NTA). Single EV analysis by Electron microscopy (TEM), Fluorescent microscopy.

Advantages: Highly pure and characterized, easy to reconstitute, easy to ship and store (+4°C), Long term storage stability (36 months).

Origin: Human urine of healthy donors
Quantity per vial: 30 µg (number of particles in 30 µg: > 1x10^8)
Storage of lyophilized exosomes: Store up to 3 years at 4°C (Expiration date indicated on the label).
Storage of reconstituted exosomes: Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.

Assay Calibration

Control (spike-in) for exosome quantification.

Protein marker analysis using different techniques.

Electron microscopy.

Flow Cytometry

Standardized positive controls for immunocapture performance evaluation.

All documents are provided in PDF format for easy viewing and printing. For additional technical documentation, please contact our support team.

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