Fluorescent EVs from HEK293 cells (double labeled with EGFP and mCherry)

FLUO-EVs are stably-fluorescent Extracellular vesicles expressing the fluorescent protein EGFP (green) and  mCherry (red) as fusion protein respectively with tetraspanin CD63 and CD9. FLUO-EVs are purified in combination of tangential flow filtration (TFF) and size exclusion chromatography (SEC) from genetically engineered cells in order to co-express the CD63-EGFP and CD9-mCherry. FLUO-EVs demonstrated high stability of the fluorophores, they can be used for in vitro tracking studies or as reference material for analyzers of nanoparticles or for assay calibration.

Characteristics: Particle size distribution 50 – 140 nm, lyophilized, storage at 4-8 °C.

Applications: Positive control for EV analyzers (NTA, flow Cytometers, fluorescence microscopy), cell spike-in and in vitro tracking, EV uptake monitoring, assay and instrument calibration.